Myskin™ case studies
Patient Profile
Mr D
9 years old
Medical Condition
40% mixed depth flame burns to trunk and lower limbs.
Position of myskin to donor graft site

Position of myskin placed over the skin graft

The Patient
After being admitted to hospital Mr D was taken to theatre to have the burnt skin excised and skin grafts, both autologous and donor, applied to the burnt areas on his legs and trunk. Although these were successful to decrease the healing time, reduce scarring and prevent further skin grafting Mr D was referred for My skin dressings.
Evaluation
For myskin treatment, a thin biopsy (approximately 0.6mm thick, 2cmx2cm) of skin is taken from the thigh area and transported to the laboratory in sterile saline solution. The biopsy is treated with a digestive enzyme overnight. The following day the keratinocytes are isolated from the dermal/epidermal junction, multiplied in cell culture and stored in liquid nitrogen until they are needed.
Three days before dressings are required, keratinocytes are thawed and cultured on a 5cm silicone disc. These discs have a patented surface layer that encourages keratinocytes, to transfer from the dressing to the wound bed and promote re-epithelialization. This cell transfer process takes about four days after which the myskin dressing can be removed and a standard dressing applied.
Ten days after being admitted to hospital the first application of myskin was applied to the skin graft donor site on his back. Five days after this the wound was assessed and showed re-epithelialisation. A week later the second application of myskin was applied to the donor sites on his legs and to the burnt area on his trunk where the original autografts were placed. Again the wounds were assessed three days after and were found to have greatly improved with rapid healing noted.
Trunk after removal of myskin dressings

Outcome
No further myskin dressings were required and Mr D made a good recovery though he continued to visit the clinic for scar management.
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